Habituation to thaxtomin A in hybrid poplar cell suspensions provides enhanced and durable resistance to inhibitors of cellulose synthesis

Table 2 Quantification of sugars in cell walls1

Cell type	Total sugars (μg mg^-1 dry wall)	Glucose (μg mg^-1 dry wall)			Uronic acids^d (μg mg^-1 dry wall)
		Total glucose	Acid-insoluble fraction	Soluble fraction^c
Non-hab	755.0 ± 113.0	445.6 ± 27.5^a	384.1 ± 15.0^a	61.5	17.1 ± 3.0^a
TA(-)hab	640.1 ± 50.0	391.9 ± 3.6^b	287.2 ± 53.1^b	104.7	31.4 ± 3.2^b

¹Total sugars, glucose and uronic acids (CDTA fraction) were quantified in dry cell walls from different cell types. Glucose was also quantified in the acid-insoluble fraction (crystalline cellulose). Samples were taken 10 days after subculture. Results are the means ± SD of three independent experiments. Cell type: Non-hab = non-habituated hybrid poplar cells; TA(-)hab = TA-habituated cells without TA.
^{a, b} Statistically different values (Student's t-test, P< 0.05) are indicated with a different letter in a column for each experiment.
^c Values were obtained by subtracting the acid-insoluble fraction values from the whole cell walls values.
^d Uronic acids were quantified from the CDTA-soluble pectin fraction.

ISSN: 1471-2229