Table 2 PCR primers used for cDNA allele cloning and amplicon sequencing, product sizes, annealing temperatures.
From: Natural diversity of potato (Solanum tuberosum) invertases
Gene | Ampli-con | Forward (F) and reverse (R) primers 5' to 3' | Length [bp] | Ta[C°] |
|---|---|---|---|---|
Pain-1 | cDNA | F: ATGGCCACGCAGTACC R: GATGAATTACAAGTCTTGCAAGGG | 1920 | 55 |
| Â | Exon 1 | F: ATGGCCACGCAGTACC R: GTTGAAAATGGTAAGCAGTTC | 360 | 52 |
| Â | Exon 3 | F: CACAAGGGATGGTATCATC R: CCCATCCCTTCTGCAG | 861 | 51 |
| Â | Exon 7 | F: CACTCAATTGTGGAGAGCTTTG R. CAAGTCTTGCAAGGGGAAGG | 201 | 59 |
InvGE | cDNA | F: ATGGAATTATTTATGAAAAGCTCTTCTCTTTGGGGGT R: TTAGTGCATCTTAGGTACATCCATGCTCCAAGC | 1761 | 55 |
| Â | Exon 1 | F: GCTCTTCTCTTTGGGGTTTAG R: TTAGGAGGTTGAAAATGAAAAC | 199 | 56 |
| Â | Exon 6 | F: GATAACTCAGTAGTGGAGAGTTTTG R: GTGCATCTTAGGTACATCCATG | Â | 56 |
InvGF | cDNA | F: ATGGATTATTCATCTAATTCTCGTTGGGCTTTGCCAG R: TCAATATTGTATCTTAGCTTTGCCCATACTCCATGC | 1743 | 55 |
InvCD141 | cDNA | F: ATGGAGATTTTAAGAAGATCTTCTTCTCTTTGGGTT R: CTAGTGCAACTTTGCATTAGCCATGCTCCAAGC | 1746 | 55 |
| Â | Exon 3 | F: GGTCCAATGTATTACAATGGAG R: GCAACTGTGATTCCTTTGATTTC | 1023 | 56 |
| Â | Exon 4 | F: GAAGTGATTTTCTCATTCACAAG R: CTTGAGGCATCAGAACACATAAG | 246 | 56 |
InvCD111 | cDNA | F: ATGGATTGTTTAAAAAAGTCTTCTC R: TCAATAAGAAGAGTGACCAAATGACCAATTCA | 1767 | 55 |