Immunolocalization of PM3 SOGG3 Proteins at the nuclear periphery. Combined antisera (zms3gsp1a and zms3gsp2) or preimmune control sera were used to stain formaldehyde-fixed uninucleate pollen mother cells. The immune complex was visualized by deconvolution microscopy in the FITC channel with A488-goat-anti-rabbit sera. Images from a single cell are shown. (A-C) Projection of the central 2/3 of the three-dimensional set of data shows DAPI image (A), FITC image (B), and pseudocolor overlay (C). Zoom up of a region of the nucleus-cytoplasm boundary is shown for the FITC (D) and overlay (E) images. Control staining with preimmune sera (F) or secondary only (G) are shown with a color scheme (red DAPI, green FITC) and scaling parameters that match those of panel C.