Figure 9From: Structure and expression of the maize (Zea maysL.) SUN-domain protein gene family: evidence for the existence of two divergent classes of SUN proteins in plantsImmunolocalization of PM3 SOGG3 Proteins at the nuclear periphery. Combined antisera (zms3gsp1a and zms3gsp2) or preimmune control sera were used to stain formaldehyde-fixed uninucleate pollen mother cells. The immune complex was visualized by deconvolution microscopy in the FITC channel with A488-goat-anti-rabbit sera. Images from a single cell are shown. (A-C) Projection of the central 2/3 of the three-dimensional set of data shows DAPI image (A), FITC image (B), and pseudocolor overlay (C). Zoom up of a region of the nucleus-cytoplasm boundary is shown for the FITC (D) and overlay (E) images. Control staining with preimmune sera (F) or secondary only (G) are shown with a color scheme (red DAPI, green FITC) and scaling parameters that match those of panel C.Back to article page