Figure 1

Experimental plan and RT-PCR analysis. (A) Scheme of the growth conditions used in this work: white rectangles (1, 3, 11) indicate the time, after the induction of Fe deficiency, at which plant root apexes were sampled only for RT-PCR semiquantitative analysis reported in (B); black rectangles (5 and 8) indicate the time at which the root apexes of Fe-deficient (-Fe) plants were sampled only for RT-PCR semiquantitative analysis reported in (B) and whole roots for the proteomic analysis. On the right, pictures of plants under the different growing conditions are shown. (B) semi-quantitative RT-PCR analysis of the genes CsFRO1 (encoding for FC-R), CsIRT1 (encoding for the IRT1) and CsHA1 (encoding for the H⁺-ATPase) in cucumber root under the different treatments. The column +Fe represents results for control plants grown in the presence of iron. The columns -Fe 1, 3, 5, 8, 11 represent results for days after -Fe treatment induction at which the roots were sampled as specified in the panel A.