Figure 3

Real time RT-PCR analysis on mRNA transcription of the differentially expressed proteins in response to A. flavus invasion under drought stress condition. Total RNA were isolated from the seeds of resistant (YJ-1) and susceptible (Yueyou7, YY7) cultivar at 50 days post-treatments. Twelve genes were selected for real time RT-PCR analysis to study the relationship between protein expression and gene transcription and the expression levels normalized using actin gene as the internal control. The expression of these genes in Yueyou 7 under well-watered conditions was used as the target calibrator. Real-time PCR analyses were performed based on three replicates.