Figure 1

2-DE analysis of peanut seed proteins from the susceptible cultivar YueyouY7 (a, b and c) and the resistant cultivar YJ-1 (d, e and f) challenged with A. flavus and drought stress(c, f), drought stress alone (b, e) and untreated as control (a, d). Proteins were separated in the first dimension on an IPG strip pH 5-8 and in the second dimension on a 15% acrylamide SDS-gel, followed by staining with Coomassie brilliant blue G-250 stain. An equal amount (200 ug) of total protein extracts was loaded in each gel. The gels were scanned and the images were analyzed using Image Master 2 D Platinum 5.0 software.