Figure 6
From: A high-throughput method for the detection of homoeologous gene deletions in hexaploid wheat
Amplification profiles of TaPFT1 homoeologues in HII mutants lacking one of the TaPFT1 homoeologous. Identification of mutants in homoeologous copies of the TaPFT1 gene in the wheat cultivar Chara using the TaqMan fluorescent probe method. (A) Strong fluorescence from FAM-labelled (TaPFT1-B specific) and NED-labelled (TaPFT1-D specific) probes, but very weak fluorescence from the VIC-labelled (TaPFT1-A specific) probe. (B) Strong fluorescence from VIC-labelled (TaPFT1-A specific), and NED-labelled (TaPFT1-D specific) probes but very weak fluorescence from the FAM-labelled (TaPFT1-B specific) probe. (C) Strong fluorescence from VIC-labelled (TaPFT1-A specific), and FAM-labelled (TaPFT1-B specific) probes, but very weak fluorescence from the NED-labelled (TaPFT1-D specific) probe.