Figure 5From: A high-throughput method for the detection of homoeologous gene deletions in hexaploid wheatTaPFT1 CAPS assay for validation. (A) Predicted CAPS digestion patterns associated with TaPFT1-D, TaPFT1-A and TaPFT1-B. Diagnostic fragments expected upon digestion with Hpy166II for TaPFT1-D (D-band), TaPFT1-A (A-band) and TaPFT1-B (B-band) are highlighted in blue boxes. Right-most panel shows the predicted restriction digestion pattern of a sample possessing all TaPFT1 homoeologues. (B) CAPS gel analysis and verification of deletion of homoeologue-specific bands from nullisomic-tetrasomic lines N5A, N5B, N5D, and Chinese Spring.Back to article page