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Figure 5 | BMC Plant Biology

Figure 5

From: A high-throughput method for the detection of homoeologous gene deletions in hexaploid wheat

Figure 5

TaPFT1 CAPS assay for validation. (A) Predicted CAPS digestion patterns associated with TaPFT1-D, TaPFT1-A and TaPFT1-B. Diagnostic fragments expected upon digestion with Hpy166II for TaPFT1-D (D-band), TaPFT1-A (A-band) and TaPFT1-B (B-band) are highlighted in blue boxes. Right-most panel shows the predicted restriction digestion pattern of a sample possessing all TaPFT1 homoeologues. (B) CAPS gel analysis and verification of deletion of homoeologue-specific bands from nullisomic-tetrasomic lines N5A, N5B, N5D, and Chinese Spring.

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