Insight into durum wheat Lpx-B1: a small gene family coding for the lipoxygenase responsible for carotenoid bleaching in mature grains

Table 1 Primer pairs used to amplify Lpx-B1 genes/alleles and transcripts, and PCR product sizes

	Forward primer (5'→3')	Reverse primer (5'→3')	Product size (bp)
*Full length*			*Gene*	*Transcript*
All Lpx-B1s	CCAAGATGATACTGGGCGGGC	CGCCGCCTTGCCGTGGTTGG	1558-4320	1154-2369
*Fragment*			*Gene*	*Transcript*
Lpx B1.1a/Lpx B1.1b/ Lpx-B1.2/Lpx-B1.3	L1, CTGATCGACGTCAACAAC^a	R1, CAGGTACTCGCTCACGTA^a	962/888/ 867/794^b	--
	GCAGGCGCTGGAAAGCAACAGGC ^c	ACTCCGCGTACTCGTCCGTCCCG ^c	1312/1238 ^b	992
Lpx B1.1a/Lpx-B1.1b	ACGCCGGTGCCGAGCGGCTC	GCGCTCTAACTCCGCGTACTCG	1106/1032^b	786
	CCACGCGGTGATGGAGCCGTTC	GCGTGTCGCGCTGACCCAGGTAC	1023/949^b	800
Lpx-B1.1c	CCAAGATGATACTGGGCGGGC ^c	CGCCGCCTTGCCGTGGTTGG ^c	1558	1154
	TACACGCCGGTGCCGAGCGGCAG ^c	CGTGTCACGCTGCCCGAGGTAGAG ^c	1137	912
Lpx B1.2	CCAGGCGGACCAGCAGGCGTGGC	ATGTACAACCGGTTGCTTTCAAGG	732	225
	TCAACGCCGGCGGCATCTTC	CGGACCGTTGCGGTTCAACAGCTG	890	794
	CCGGTGCCGAGCGGCTCCATG ^c	CGGTTCGGGAGGAACCCCGCGTAG ^c	871	729
Lpx-B1.3	GCCGGCGGTGCGCACATACGTG	GTCTGCCGCGTACGGGTAGTCCTC	1585	1056
	GGTAGGCTCACGCCGCTCGCCAC	GCACCGGGTGCGTCACGCTGAGG	339	256
17S rRNA	GCTCGTAGTTGGACCTTGG ^c	GTATCTGATCGTCTTCGAGC ^c	--	382

^aThis is the LOXA-L1/R1 primer pair previously used by Carrera et al. [10]. ^bThe different amplicon sizes are due to the length polymorphism of the MITE in the intron region. ^cPrimer pairs highlighted in bold were those used for Figures 3B and 5.

ISSN: 1471-2229