Figure 4

Analysis of the deduced amino-acid sequences of durum wheat LOX-1s. (A) Protein sequence alignments between barley LOX-1 (HvLOX-1) [GenBank: L35931] and durum wheat LOX-1s deduced from the expressed sequences. Conserved domains (from I to V) as assigned by Minor et al. [17] are underlined. Asterisks indicate residues required for iron binding. The 9-LOX-specific TV/R motif is boxed. The alignments were performed using Vector NTI AlignX software (Ver. 9.0, Invitrogen). (B) N-terminal PLAT domain and typical LOX domain in both the complete and deleted variants of durum wheat LOX-1s, as identified by the NCBI Conserved Domain Search Database (http://www.ncbi.nlm.nih.gov/Structure/cdd/cdd.shtml).