Figure 3
Chromosomal mapping and analysis of the distribution among the genotypes of the Lpx-B1 genes and alleles. (A) The genetic linkage groups of chromosome 4BS from Creso × Pedroso and Ofanto × Cappelli RIL populations. The genetic distances of markers are indicated in cM to the left of the chromosome bars. (B) Distribution of the Lpx-B1 genes and alleles in 18 durum wheat cultivars of the germplasm collection. Representative gel separation of genomic DNA extracted from leaves and amplified using gene and allele specific primer pairs (highlighted in bold in Table 1), according to conditions described in Methods. MAT, Matarese; TRI, Trinakria; KIP, Kiperounda; GRI, Grifoni; TIM, Timilia; CAP, Capeiti 8; PRI, Primadur; BRI, Brindur; TRE, Tresor; CLA, Claudio; NEO, Neodur; VIT, Vitromax; COS, Cosmodur; CRE, Creso; OFA, Ofanto; FOR, Fortore; VAR, Varano; LES, Lesina. The gel separation is in inverted colours.