Figure 6

RNA gel blot analysis of Na⁺ transport system genes in Thellungiella and Arabidopsis. Total RNA was prepared from 3-week-old Thellungiella and Arabidopsis plants subjected to 250 mM NaCl stress treatment for the periods indicated. Each lane was loaded with 10 μg total RNA. The probes were the following full-length cDNA fragments from Thellungiella and Arabidopsis, respectively: SOS1 (AK353556), NHX1 (AK352788), NHX2 (AK353509), NHX5 (AK353024), and HKT1 (AK353477).