Figure 5

Effect of salt stress on transcript levels of ThSOS1 and ThSOS1S. (A) Schematic of the corresponding regions of the primers in ThSOS1, ThSOS1S and AtSOS1 for RT-PCR analysis. Exons are indicated as filled boxes and introns as spaces between them. (B) Relative expression of ThSOS1 and ThSOS1S under salt stress. Two-week-old Thellungiella seedlings were exposed to 250 mM NaCl stress for 0, 5, or 10 h. Total RNA was reversed-transcribed into cDNA and used as a template for quantitative RT-PCR. The qRT-PCR performed using blue-primer sets in (A). Relative transcript levels were normalized to Actin2 mRNA. Data are the mean ± SD for three individual experiments (n = 3). (C) Semiquantitative gene expression of SOS1 splice variants in Thellungiella and Arabidopsis under normal growth conditions. The RT-PCR performed using red-primer sets in (A), which were able to detect these splice variants.