Figure 4

Comparison of the structures of ThSOS1 and ThSOS1S. (A) Alignment of the transmembrane regions of NHE1 (P19634), SynNhaP (D90910), SOS1 (AF256224), and ThSOS1 (EF207775.1) and the complete sequences of NhaP (P13738) and ThSOS1S constructed with ClustalW ver. 1.83. Amino acid residues conserved in all sequences are highlighted in black, and conserved substitutions are shown in gray. Predicted membrane spanning regions (TM) were determined by TMpred [42] and are marked under the alignment. (B) Close-up alignment of the nucleotide sequences of ThSOS1, ThSOS1S, and the genome with the deduced amino acid sequences from Gly-435 to Asp-479. The nucleotides conserved in ThSOS1 and ThSOS1S are written in capital letters, and the ThSOS1S-specific sequence is written in lower-case letters. Intron sequence is shown in gray letters. The predicted amino acid sequence of ThSOS1 is shown in capital letters, and that of ThSOS1S in lower-case letters. The 19aa insert of ThSOS1S is red-underlined. The asterisk shows the point at which the ThSOS1S protein is truncated. (C) Hypothetical secondary structure models of ThSOS1 and ThSOS1S.