Expression profiles of miRNAs in sugarcane organs/tissues. (A) Stem-loop pulsed RT-PCR of SsmiRNAs in organs/tissues of sugarcane hybrid RB 83-5486. (B) Stem-loop pulsed RT-PCR of SsmiRNAs in organs/tissues of the S. officinarum and S. spontaneum. Saccharum spp. GAPDH (SsGAPDH; TC77224) was used as a loading control. RT- (reaction without RT) and PCR- (reaction without cDNA) are shown as negative controls. Numbers in parentheses represent PCR cycles for each amplicon.