New sorghum miR827 precursor is highly similar at sequence and secondary structure levels with its sugarcane homolog. (A) Partial multiple sequence alignment of miR827 precursors from Saccharum spp (Ss), Sorghum bicolor (Sb), Oryza sativa (Os), Zea mays (Zm), Brachypodium distachyon (Bd), Arabidopsis thaliana (At), and Populus trichocarpa (Pt). Sequence alignment was performed using the T-Coffee program . Black line on top of the alignment indicates the mature miRNA sequence. (B) Conserved secondary stem-loop structures of pre-miR827 in sugarcane (Ss) and sorghum (Sb). Red line marks the mature miR827 sequence.