Figure 4

Cellular localization of iron in the P. vulgaris genotype G14519. Beans soaked for 18 hours (a and e to h) or unsoaked (b to d) stained with Perls' Prussian blue reagent. a,d,g: Stereomicroscopy. a: Cotyledon tissue, close-up of provascular bundle (pvb). d: Longitudinal section of embryonic axis (axis) including cotyledon tissue (cot), and the axis attachment zone indicated by a filled arrow. g: Seed coat, showing the inside (ins) part toward the cotyledons and the stain at the outer side (os) of this tissue. b, c, f and h: light microscopy of thin sections of PPB stained tissue. b and c: Close-up of cotyledon pvb, surrounded by blue stained bundle-sheath-like cells which contain amyloplasts (am). e: Close-up of radicle meristem showing the stained meristematic cells (mer). f: Section of hypocotyl tissue showing iron accumulation in the cells proximal to the epidermis (ep). h: Seed coat section illustrating blue stain in the palisade layer (pl). Scale bars in d and g: 1 mm, in a, b, e, f, and h: 0.1 mm, and in c: 0.01 mm.