Figure 3

Number of 454 pyrosequence reads representing gene transcripts corresponding to known benzylisoquinoline alkaloid biosynthetic enzymes . The cDNA library used for 454 pyrosequencing was prepared from opium poppy cell cultures treated with a fungal elicitor for 10 h. Sequence counts include unigenes encoding predicted proteins with > 90% amino acid sequence identity to known opium poppy enzymes except for CheSyn and StySyn, which were compared with known enzymes from Eschscholzia californica. Black bars represent unigenes encoding enzymes involved in the conversion of precursor tyrosine to the central intermediate (S)-reticuline. Red bars refer to unigenes encoding enzymes involved in the formation of sanguinarine, blue bars represent unigenes encoding enzymes involved in the biosynthesis of morphine, and green bars correspond to other enzymes with a role in benzylisoquinoline alkaloid metabolism. Abbreviations are as indicated in Figure 1 and Additional File 1.