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Table 3 List of gene products for the 50 most abundant 454 transcript reads in the normalized cDNA library from bitter melon seeds.

From: Mining the bitter melon (momordica charantial.) seed transcriptome by 454 analysis of non-normalized and normalized cDNA populations for conjugated fatty acid metabolism-related genes

 

Normalized cDNAs

Non-normalized cDNAs

Homolog ID

# of

Reads

# of

Contigs

At Gene

Identifier

Gene Products

# of

Reads

# of

Contigs

gi|11225518

3,291

6

-

MAP30

8,197

45

gi|29243138

2,615

3

-

hypothetical protein

15,142

34

gi|19150

2,110

1

-

b-luffin

0

0

gi|13171073

1,701

1

-

ribosome-inactivating protein precursor

6,309

3

gi|21327881

1,928

12

-

napin [bitter melon charantia]

23,678

169

gi|3808062

632

1

At3g22640

cupin family protein

4,278

42

gi|29165641

618

1

-

trichosanthin precursor

1,804

5

gi|4106063

584

3

-

Trichoanguin

1,282

8

gi|147798373

767

4

At1g49320

BURP domain-containing protein

5,370

10

gi|157335527

414

2

At1g45201

similar to lipase class 3 family protein

1,000

3

gi|976231

460

2

At2g02990

ribonuclease (RNase LC1)

2,438

3

gi|170444

374

1

At2g02490

Extension (class II)

780

1

gi|157342180

274

1

At1g19100

ATP-binding region

41

1

gi|73808794

248

1

At4g09320

NDPK1 (nucleoside diphosphate kinase 1)

214

2

gi|18479082

238

1

At4g28520

11 S globulin-like protein

151

15

gi|84514139

231

1

At3g14610

CYP72A7; cytochrome P450

230

6

gi|157337377

230

1

At5g59750

riboflavin biosynthesis protein, putative

102

8

gi|157336060

219

1

At1g77460

C2 domain-containing protein

26

1

gi|118482397

202

1

At1g75270

DHAR2; glutathione dehydrogenase

833

3

gi|1838961

202

2

At4g25050

ACP4 (Acyl carrier protein 4)

714

2

gi|74229677

196

1

At1g08830

CSD1 (copper/zinc superoxide dismutase 1)

73

1

gi|162672646

170

1

-

predicted protein

0

0

gi|17402469

167

1

At4g14960

TUA6 (tubulin alpha-6 chiain)

46

2

gi|157350920

162

1

At1g07040

similar to unknown protein

12

2

gi|118481513

152

1

At2g36620

RPL24A

0

0

gi|157352507

142

1

At1g61580

ARP2/RPL3B

40

1

gi|157327297

156

2

At1g18540

60 S ribosomal protein L6 (RPL6A)

212

3

gi|118482623

131

1

At2g02130

LCR68/PDF2.3 (LMW cysteine-rich 68)

637

2

gi|37721219

127

1

AtCg00900

ribosomal protein S7

0

0

gi|77999293

157

2

At5g02960

40 S ribosomal protein S23 (RPS23B)

154

2

gi|145910320

126

1

At5g35360

acetyl co-enzyme A carboxylase subunit

24

3

gi|1762945

125

1

At4g14420

lesion inducing protein-related

222

1

gi|118484529

142

2

At3g53740

60 S ribosomal protein L36 (RPL36B)

266

4

gi|13430182

141

2

At3g04400

ribosomal protein L17

172

1

gi|157342677

123

1

At4g29520

similar to Saposin B

44

4

gi|21304463

121

1

AtCg01310

chloroplast ribosomal protein L2

80

2

gi|157344877

119

1

At1g72150

PATL1 (PATELLIN 1); transporter

0

0

gi|49617323

119

1

At3g27660

OLEO4 (OLEOSIN4)

28

1

gi|2204236

121

2

At2g05990

Enoyl-[acyl-carrier-protein] reductase

0

0

gi|20514369

118

1

At4g35160

O-methyltransferase family 2 protein

59

1

gi|118482052

118

1

At3g05560

structural constituent of ribosome

0

0

gi|157340503

129

2

At1g75760

ER lumen protein retaining receptor family

194

1

gi|157358487

138

2

At1g27330

similar to unknown membrane protein

1121

6

gi|71842524

137

3

At1g70580

AlaT1

97

1

gi|157339643

152

3

At5g55190

RAN3; GTP binding

188

4

gi|13899097

108

1

At5g21090

leucine-rich repeat protein, putative

0

0

gi|38093741

107

1

At1g31812

ACBP (ACYL-COA-BINDING PROTEIN)

4

1

gi|118482799

106

1

At1g08830

CSD1 (copper/zinc superoxide dismutase 1)

50

1

gi|157343130

150

2

At4g30810

SCPL29 (serine carboxypeptidase-like 29)

13

2

gi|1666094

103

1

At5g51810

gibberellin 20-oxidase

402

4

  1. Numbers of reads and contigs and most related homolog for each gene product are also shown. For comparison, the numbers of read and contigs for each transcript in the non-normalized cDNA library are indicated.