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Figure 8 | BMC Plant Biology

Figure 8

From: Mining the bitter melon (momordica charantial.) seed transcriptome by 454 analysis of non-normalized and normalized cDNA populations for conjugated fatty acid metabolism-related genes

Figure 8

Characterization of FAD2 polypeptides and genes in bitter melon. (A) Alignment of amino sequences for divergent FAD2 s (McFAD2, McFAD2v) and the FAD2-related conjugase (McConj) from bitter melon cDNA library. Sequences were aligned by Clustal X and displayed using GeneDoc Software. (B). Phylogenetic tree of FAD2 s and conjugase. Sequences used to construct the tree include Arabidopsis thaliana At FAD2 (gi 21536781), Trichosanthes kirilowii Tk FAD2 (gi 28371823), Tk Conj (gi 28371821), and Sorghum bicolor Sb DES2 (gi 242062720). (C). Production of 16:2 and 18:2 in Saccharomyces cerevisiae by expression of McFAD2 and McFAD2v. As indicated, no 16:2 or 18:2 was detected in yeast cells containing only the vector. (D) Expression of McFAD2, McFAD2v, and conjugase (McConj) during bitter melon seed development as determined by RT-PCR. β-Tubulin (β-Tub) -specific primers were used as a control to assess the quality of first-strand cDNA.

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BMC Plant Biology

ISSN: 1471-2229

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