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Figure 1 | BMC Plant Biology

Figure 1

From: Mining the bitter melon (momordica charantial.) seed transcriptome by 454 analysis of non-normalized and normalized cDNA populations for conjugated fatty acid metabolism-related genes

Figure 1

Fatty acid compositions of lipids and detection of selected transcripts at different stages of bitter melon seed development. (A). Distribution of fatty acids in phospholipids (PL, open circle) and the triacylglycerol (TAG)-enriched neutral lipids (NL, black square) during the development of bitter melon. The fatty acids shown are palmitic acid, 16:0; stearic acid, 18:0; oleic acid, 18:1; linoleic acid, 18:2; linolenic acid, 18:3; and eleostearic acid, ESA. (B). RT-PCR detection of transcripts for the bitter melon conjugase (McConj) and oleosin (McOleo) during bitter melon seed development. β-Tubulin (Mcβ-Tub2)-specific primers were used as a control to assess the quality of first-strand cDNA. The seeds were collected at the designated days after pollination (DAP).

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