Sequencing of 6.7 Mb of the melon genome using a BAC pooling strategy

BMC Plant Biology

Table 5 C. melo BAC sequences characteristics^a.

Total sequence length	6,230,040 bp
Sequence length excluding stretches of Ns	5,958,994 bp
Number of predicted protein coding genes ^b	616
Number of predicted protein coding genes with homology to *C. melo* ESTs	451 (73.2%)
tRNA genes	11
Gene density ^c	9.9 genes/100 kb (1.5 - 19.7, SD: 4.3)
Average exon length	238 bp
Average intron length	393 bp
Exons per gene	4.9 (1-29, SD: 4.4) (74% of genes ≤ 6 exons) (23% intronless)
Average protein length ^d	386 (34-2,156, SD: 268)
Average% of introns in coding sequence ^e	45.6 (4.3 - 95.5, SD: 20.6)
GC content (%)	33 (30.2 - 38.7, SD: 1.34)
SSRs ^f	4,430 (74,590 bp, 1.25% of total sequence) 1 SSRs/1.3 Kb
Transposable elements ^g	139

^aFrom the analysis of all 57 scaffolds plus one contig longer than 20 kb
^bGenes from transposons not counted
^cPartial genes at BAC borders counted as 0.5 genes
^dTransposon proteins not considered
^e ORFs without introns not considered
^fMinimum repeat lengths considered: 10 bp (mononuc.), 12 bp (di- and trinuc.), 16 bp (tetranuc.), 20 bp (pentanuc.) and 24 bp (hexanuc.)
^gSee Table 6 for a more detailed analysis of transposon content

ISSN: 1471-2229