Schematic representation of the relational pipeline used to build the miSolRNA Database. BAC and Unigenes sequences were downloaded from the SOL Genomic Network database(1) and Solanum Lycopersicum (Sly) and Arabidopsis thaliana (Ath) miRNAs from the miRBase(2). Putative miRNA target sites were searched using the miRANDA software as described in the text. Recognized sequences were scored and filtered base on penalties mismatches. Both Unigenes and full length BAC sequences were positioned onto the different BINs of the S. pennellii IL population map(3) by searching all mapped markers using the on-line comparative map viewer tool described by Mueller et al, . Expression data of targeted Unigenes were retrieved from a previously described microarray experiment performed along developmental and ripening processes of tomato fruit (4). Quantitative Metabolic Loci (QML) data previously detected by Schauer et al  co-localizing with targeted BIN were integrated into the relational database(5). Hits were defined as the annotations of Unigenes available at the SGN data base and by de novo performed by Augustus, Genome Threader and Arabidopsis BLASTX annotations(6). Genomic clones of Sly miR precursors were searched by BLASTN against the miRBase sequence DB as described in the text(7). The entire information was incorporated into the miSOLRNA database and made available through a dedicated web interface.