Figure 6
Northern analysis of selected stress responsive genes. Northern analysis showing expression of ten selected stress responsive genes [Aquaporin like water channel protein; GenBank: FL512354, Metallothionein; GenBank: FL512338, Proline rich protein; GenBank: FL512352, P type H+ATPase; GenBank: CD051280, Putative ABA response protein; GenBank: FL512397, LEA protein 2; GenBank: CD051326, β-amylase; GenBank: CD051266, Zn finger protein; GenBank: FL512439, Dehydration responsive element bp3; GenBank: FL512463 and Elongation factor 1 alpha; GenBank: FL518919] in PUSABGD72 and ICCV2. 20 μg of total RNA isolated from control/stressed seedlings of PUSABGD72/ICCV2 were separated in formaldehyde denaturing gel, transferred to nylon membrane and probed with α32P-dCTP labeled amplified cDNA fragments corresponding to indicated EST clones. An amplified product of chickpea Actin cDNA was used as an internal control and 28S ribosomal RNA was shown as loading control. Time points in days (d) are indicated.