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Table 2 Origin of the genes and the primers for the RT-PCR experiments.

From: The development and evaluation of single cell suspension from wheat and barley as a model system; a first step towards functional genomics application

Name

Primers

Tubulin

F2 5' CCTCATCACCGTCCTCGCC 3'

R2 5' TGATCTCAGCTGAGAAGGC 3'

P5CS

F1 5' CGTGAAGCGCATCATAATCA 3'

R1 5' ATCAGCCCACTCTGACCAAC 3'

P5CR

F1 5' AACGAACCCTTCTCGAGCTCATG 3'

R1 5' AGCAGCATCAGTGATGTGTCTAG 3'

  1. For the primer design NCBI gene bank entries were used (tubulin: T. aestivum tubulin 3A DQ435663, H. vulgare α-tubulin 3 AJ132399; P5CS T. aestivum AB 193551 and H. vulgare AK249154; P5CR: T. aestivum AAW82908 and H. vulgare AY177684).
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BMC Plant Biology

ISSN: 1471-2229

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