Figure 5

Dehydration induced genes show broader regions of H3K4me3. The median value of H3K4me3 coverage was calculated at positions along the genes for three different sets of genes. Each set was further limited to contain only genes between 1,000 to 2,000 bp in length. The three sets of genes were: all expressed genes, all dehydration induced genes, and all dehydration induced genes also known to be ABA regulated. These groups are indicated by the color key at upper right of the Figure. The median level of H3K4me3 observed for each set of genes (y-axis, Median coverage) was determined at different positions along the genes (x-axis in bp, with genes normalized to a standard 1500 bp size between the TSS and STOP codon and containing 500 bp upstream or downstream from these sites). (A) The actual coverage profiles without peak normalization. (B) Profiles that were normalized for peak heights to facilitate comparison of the profile shapes. The p-values for the normalized profiles for the dehydration/ABA induced genes in (B), which measure the probability of the broader profiles occurring by chance, were p = 0.002 for the watered condition and p = 1.4 × 10^-10 for the water deficit condition.