Genetic and environmental control of the Verticillium syndrome in Arabidopsis thaliana

Häffner, Eva; Karlovsky, Petr; Diederichsen, Elke

doi:10.1186/1471-2229-10-235

BMC Plant Biology

Table 2 QTL detected for Verticillium-related traits in the A. thalian a (Bur×Ler) F2/F3 mapping population.

From: Genetic and environmental control of the Verticillium syndrome in Arabidopsis thaliana

Trait	QTL	Exp.	Chr.	Peak pos. (cM)	Nearest marker	LRS	LOD	Explained trait variance	Mean ± SD (or median) for F3-families with alleles from:
									Bur	Heterozygous	Ler
Degree of Verticillium colonisation (vec; malt agar test; number of colonised shoot segments/total number of shoot segments in%)	vec1	E1	2	26	erecta	21.2**	4.6	31%	20.4 ± 13.8^a	18.4 ± 14.5^a	43.8 ± 27.1^b
	vec2	E2	4	10	nga8	9.4*	2.0	11%	21.2 ± 17.2^a	26.3 ± 21.8^a	41.8 ± 15.8^b
	vec3	E3	4	45	ciw7	17.0**	3.7	18%	32.0 ± 29.2^a	41.0 ± 20.5^a	65.2 ± 21.5^b
	vec4	E1	5	11	nga139	20.7**	4.5	31%	7.7 ± 6.0^a	23.2 ± 16.5^b	42.0 ± 24.1^c
Degree of Verticillium colonisation (vec a; real-time qPCR; pg Verticillium DNA/100 mg shoot fresh weight)	vec1 a	E3	2	27	erecta	14.2**	3.1	20%	764 ± 1071^a	1061 ± 1035^a	1994 ± 1588^b
	vec3 a	E3	4	47	ciw7	13.4*	2.9	13%	976 ± 1162^a	751 ± 741^a	2015 ± 1615^b
Development time (dt; days from germination to maturity in mock-inoculated plants)	dt1	E1	4	5	nga8	18.8**	4.1	29%	64.4 ± 7.5^a	58.6 ± 7.4^a,b	54.3 ± 3.8^b
	dt2	E1	4	16	nga8	18.2**	4.0	28%	64.4 ± 7.5^a	58.6 ± 7.4^a,b	54.3 ± 3.8^b
	dt3	E1	4	33	ciw6	17.2**	3.7	26%	63.3 ± 7.2^a	58.0 ± 7.2^a,b	54.5 ± 4.7^b
	dt4	E2	5	5	nga151	17.0**	3.7	24%	50.3 ± 3.1^a	55.0 ± 4.8^b	49.8 ± 5.7^a
Stunting resistance (stre; shoot height of Verticillium-inoculated plants in cm)	stre1	E1	1	79	nga111	32.5***	7.1	51%	10.9 ± 2.2^a	16.1 ± 3.8^b	20.2 ± 4.3^c
	stre2	E1	1	59	nga128	22.2**	4.8	39%	11.1 ± 2.3^a	17.4 ± 4.5^b	17.8 ± 3.9^b
	stre3	E3	5	31	SO191	20.4**	4.4	39%	28.7 ± 3.1^a,b	24.7 ± 5.3^a	32.7 ± 3.5^b
	stre4	E3	5	39	SO191	20.9**	4.5	40%	28.7 ± 3.1^a,b	24.7 ± 5.3^a	32.7 ± 3.5^b
	stre5	E3	5	53	nga129	15.8**	3.4	32%	26.5 ± 3.1^a	25.7 ± 5.5^a	33.5 ± 3.2^b
Verticillium-induced axillary branching (vab; number of plants with a branching score above the overall median of Verticillium-inoculated plants)	vab1	E1	1	9	F16J7-	28.1***	6.1	39%	20^a	13^b	10^b
					TRB
	vab2	E1	1	34	EH1-2	22.2**	4.8	33%	20^a	12^a,b	10.5^b

LRS = likelihood ratio statistic. Asterisks denote genome-wide significance thresholds determined by permutation tests as follows: * 37%, ** 95%, ***99.9%. In each row, values with different letters differ significantly with at least p < 0.05. ANOVA and tukey tests were used for metric values; differences of values that were not normally distributed were confirmed with pairwise Mann-Whitney-U-tests. For axillary branching scores the median is given; values were compared pairwise with U-tests. Results are displayed for the most representative experiment (exp.). For reproducibility see Additional file 4.

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ISSN: 1471-2229

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