Figure 5

Deep sequencing reads for C. sativa agmitine deiminase. Sequences identified from 454 sequencing of cDNA from 15 DPA C. sativa seeds, aligned with the Arabidopsis agmatine deiminase cDNA (At5G08170). The three haplotypes are evident and indicate that three different copies of this "single-copy gene" are present and expressed in the C. sativa genome, presumably representing three homoeologs. Similar results were obtained with the other 5 single-copy genes sampled (Additional File 7). The degree of nucleotide similarity estimated in these aligned regions (3-6%) is consistent with the diversity observed in FAD2 and FAE1 loci.