FAD2 and FAE1 expression in developing seeds. Relative combined expression of all three copies of (A) CsFAD2 and (B) CsFAE1 measured by real time qPCR at 15, 20, 25, and 30 days post anthesis (DPA) and in 2 week old seedlings. The 20 DPA sample, which expressed CsFAD2 and CsFAE1 at the highest amount, was arbitrarily set to 100% and used as the calibrator for the remaining samples. Error bars represent the standard deviation of 3 replicate experiments. Sequenom SNP analysis demonstrating the expression of each version of (C) CsFAD2 or (D) CsFAE1 relative to the other versions. Error bars represent the standard deviation of three (for CsFAD2) or four (for CsFAE1) SNP analyses. Because FAE1 is not expressed in C. sativa seedlings (B), the relative expression of the 3 copies of CsFAE1 in seedling tissue is not shown (D).