Table 7 Sequence of PCR primers used for quantification of different S. lycopersicum PAL genes using RT-PCR.
Primer name | Sequence (5'-3') | Target | Accession number | Fragment length (bp) |
|---|---|---|---|---|
LE02F | AGTGGCAACCCTTTAATTCG | S. lycopersicum; PAL1 | TC153702 | 479 |
LE02R | CATGTCATCATGTTCACAAAGC | Â | M83314 | Â |
LE415F | TGAAGGAATGGAATGGTGCT | S. lycopersicum; PAL2 | TC165415 | 303 |
LE415R | TGAAAGAAGCCACAAAAGTTCA | Â | Â | Â |
LE86F | CAGAATTAAAGGCCGTGTTG | S. lycopersicum; PAL3 | TC153686 | 295 |
LE86R | TTTCTGGCAAGCATCTAGCA | Â | Â | Â |
LE99F | CGGTGAGGAGATTGACAAGG | S. lycopersicum; PAL4 | TC153699 | 199 |
LE99R | CCTGTAAAGTTGTAGAAATTGAATGAA | Â | Â | Â |
LE88F | GGTTGGTTAGACAAGAAGTTGGA | S. lycopersicum; PAL5 | TC153688 | 404 |
LE88R | TGTCGTAGTGGGCGTGATTA | Â | M90692 | Â |
LE67F | TTGCAAACAGGATCAACGAA | S. lycopersicum; PAL6 | TC165267 | 220 |
LE67R | TTGCTTCACTTCACTTCTAACAGACTGG | Â | Â | Â |
LEbtubF | GGGTAAGATGAGCACAAAGGA | S. lycopersicum; β-tubulin | TC153831 | 440 |
LEbtubR | GGCAGAAATTGAACAAACCAA | Â | Â | Â |