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Table 2 HPLC analyses of phenylpropanoids.

From: The Ve-mediated resistance response of the tomato to Verticillium dahliae involves H2O2, peroxidase and lignins and drives PALgene expression

  

Phenolic content (μg g-1 FW)

  

Ferulic acid

p-Coumaric acid

Vanillin

p-Hydroxybenzaldehyde

  

LA3030

LA3038

LA3030

LA3038

LA3030

LA3038

LA3030

LA3038

2 hpi

C

2.63 ± 0.36

2.59 ± 0.01

0.22 ± 0.02

0.54 ± 0.01

0.14 ± 0.01

0.09 ± 0.01

0.49 ± 0.03

0.48 ± 0.01

 

I

3.50 ± 0.01

5.16 ± 0.29*

0.41 ± 0.06

0.27 ± 0.01

0.24 ± 0.01

0.26 ± 0.01

0.40 ± 0.01

0.42 ± 0.05

4 hpi

C

3.39 ± 1.12

2.68 ± 0.01

0.43 ± 0.05

0.33 ± 0.09

0.20 ± 0.01

0.21 ± 0.01

0.43 ± 0.04

0.38 ± 0.02

 

I

2.31 ± 1.63

3.34 ± 0.93

0.38 ± 0.15

0.37 ± 0.07

0.20 ± 0.01

0.24 ± 0.01

0.37 ± 0.01

0.43 ± 0.01

8 hpi

C

2.75 ± 0.49

2.91 ± 0.14

0.42 ± 0.02

0.48 ± 0.01

0.21 ± 0.01

0.20 ± 0.01

0.39 ± 0.04

0.37 ± 0.01

 

I

2.34 ± 1.62

3.30 ± 0.93

0.51 ± 0.09

0.44 ± 0.18

0.20 ± 0.09

0.24 ± 0.05

0.38 ± 0.08

0.48 ± 0.02

16 hpi

C

1.89 ± 0.12

3.42 ± 0.12

0.20 ± 0.19

0.46 ± 0.01

0.16 ± 0.01

0.15 ± 0.01

0.25 ± 0.09

0.38 ± 0.01

 

I

3.22 ± 0.22

2.92 ± 0.13

0.35 ± 0.14

1.00 ± 0.29*

0.20 ± 0.01

0.19 ± 0.02

0.34 ± 0.05

0.54 ± 0.03

24 hpi

C

2.94 ± 0.03

2.95 ± 0.43

0.10 ± 0.02

0.51 ± 0.05

0.14 ± 0.01

0.23 ± 0.01

0.32 ± 0.07

0.33 ± 0.06

 

I

3.68 ± 0.17

3.56 ± 0.31

0.41 ± 0.11

1.01 ± 0.12*

0.22 ± 0.03

0.27 ± 0.01

0.37 ± 0.07

0.85 ± 0.07*

48 hpi

C

2.85 ± 0.03

3.61 ± 1.39

0.63 ± 0.46

0.60 ± 0.23

0.15 ± 0.01

0.20 ± 0.01

0.35 ± 0.07

0.49 ± 0.02

 

I

1.88 ± 0.95

3.33 ± 1.29

1.01 ± 0.68*

0.92 ± 0.40*

0.17 ± 0.01

0.27 ± 0.06

0.52 ± 0.04*

0.97 ± 0.01*

96 hpi

C

3.65 ± 0.25

3.25 ± 0.21

0.40 ± 0.02

0.46 ± 0.30

0.23 ± 0.01

0.21 ± 0.02

0.31 ± 0.03

0.44 ± 0.02

 

I

4.85 ± 0.09

4.57 ± 0.50

1.51 ± 0.34*

0.61 ± 0.14*

0.23 ± 0.01

0.43 ± 0.02*

0.57 ± 0.04*

1.61 ± 0.01*

192 hpi

C

3.31 ± 0.01

3.44 ± 0.17

0.57 ± 0.27

0.40 ± 0.35

0.20 ± 0.01

0.21 ± 0.03

0.38 ± 0.01

0.37 ± 0.08

 

I

3.46 ± 0.26

4.48 ± 1.68

0.66 ± 0.46

0.45 ± 0.22

0.22 ± 0.05

0.34 ± 0.02*

0.38 ± 0.01

0.80 ± 0.07*

  1. HPLC analysis of ferulic acid, p-coumaric acid, vanillin and p-hydroxybenzaldehyde contents in the cell wall-bound fraction of control and V. dahliae-inoculated resistant (LA3038) and susceptible (LA3030) tomato roots. The results are the mean values of three independent assays. Hpi: hours post-inoculation; C: control plants; I: inoculated plants; FW: fresh weight. Means followed by * are significantly different from the control group mean for that time point (P < 0.05).