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Table 3 Primers used in chimeric expression vector construction

From: Mitochondrially-targeted expression of a cytoplasmic male sterility-associated orf220 gene causes male sterility in Brassica juncea

Genes

F/R

Primers Sequence (5'-3')

orf220

F

ATGCCTCAACTGGATAAATTCACTT

 

R

TCATCGAAATAGATCGAGGATCTCG

Chimeric gene orf220

F

TACGGATCCATGCCTCAACTGGAT (BamHI)

 

R

AGAGAGCTCTCATCGAAATAGATC (SacI)

Chimeric gene atp2-1

F

CCTCTAGACCATGGCTTCTCGGAGGCTTCT (XbaI)

 

R

CCGGATCCGCTGCGGAGGTAGCGTACTG (BamHI)

atp2-1/orf220 GFP

F

CACCATGGCTTCTCGGAGGCTTCT

 

R

TCGAAATAGATCGAGGATCTCG

orf220 GFP

F

CACCATGCCTCAACTGGATAAATTCACTT

 

R

TCGAAATAGATCGAGGATCTCG

  1. Note: underlined italicized nucleotides represent restriction enzyme sites. F, forward; R, reverse.