Genes | Primers | Annealing temperature (°C) | Product size (bp) | Sequences (5'-3') |  | |
---|---|---|---|---|---|---|
 |  |  |  | Forward | Reverse | Used for |
PtrABF | GSP1 | 60 | 1511 | ATTGGAGCAAGCTGTTGCCACCGC | CGCAGCTGCCTACACTCCATGG | Amplification of full-length cDNA |
Actin | Actin | 58 | 107 | ATTGTAAGCAACTGGGATGATA | AGAGGTGCCTCAGTGAGAAG | Quantitative real-time PCR |
PtrABF | GSP2 | 57 | 113 | CCATCACCAGTTCCTTATGTGTT | GATCTTGCAGCTGACTCTCTGTT | Â |
PtrABF | GSP3 | 58 | 1363 | GGCTCGAGATGGGATCTCAAATGAACTTCAAG (XhoI site is underlined) | AAGGATCCCGTCAGTGTCCTCCTCAAGCACAG (BamHI site is underlined) | Subcellular localization |
GFP | GFP-GSP1 | 60 | 732 | CGGGATCCATGAGTAAAGGAGAAG (BamHI site is underlined) | CGGTACCTTATTTGTATAGTTCATCC (KpnI site is underlined) | Â |
PtrABF | GSP4 | 60 | 1366 | GGCCATGGCGATGGGATCTCAAATGAACTTCAAG (NcoI site is underlined) | ATAGGATCCCTACCAAGGGCCCGTCAGTGTCC (BamHI site is underlined) | Transcriptional activation assay |
PtrABF | GSP5 | 58 | 160 | ATTGGAGCAAGCTGTTGCCACCGC | CGCAGCTGCCTACACTCCATGG | Transgene overexpression |
CaMV35S-PtrABF | 35S-PtrABF | 60 | 2013 | CGCCGTAAAGACTGGCGAACAGTTCATACAGAGT | CGCAGCTGCCTACACTCCATGG | Confirmation of transgenic plants |
NPTII | NPTII | 56 | 742 | AGACAATCGGCTGCTCTGAT | TCATTTCGAACCCCAGAGTC | Â |
NtADC1 | Â | 58 | 306 | CTTGCTGATTACCGCAATTTATC | CCTTACTGCAGGCTTTTCATCTA | Analysis of expression levels |
NtADC2 | Â | 58 | 350 | GCCGGCCCTAGGTTGTTGTGTAGATG | AGCGAACAACAAGAGGCAGCTGAAGCC | Â |
NtAREB | Â | 58 | 224 | TCTTCACAGCAAAAGCCTCA | GTGACCCCATTATGCAATCC | Â |
NtCAT | Â | 58 | 151 | AGGTACCGCTCATTCACACC | AAGCAAGCTTTTGACCCAGA | Â |
NtCDPK2 | Â | 58 | 267 | AGGTGAGCTTTTCGATAGGATTATT | ACTTCTGGTGCAACATAGTAAGGAC | Â |
NtDREB | Â | 58 | 503 | GGACCCACTTGCTGATTCTT | GCGCCTCCTCATCCATATAA | Â |
NtERD10C | Â | 58 | 366 | ACGTGGAGGCTACAGATCGTGGTTTG | TCTCCACTGGTACAGCCGTGTCCTCAC | Â |
NtERF | Â | 58 | 220 | ACACTTCATTTTCACATTCCAACTT | TCTTCTAATTCTTGACCATGGCTAC | Â |
NtLEA5 | Â | 58 | 350 | TTGTTAGCAGGCGTGGGTAT | CTCTCGCTCTTGTTGGGTTC | Â |
NtAPX | Â | 58 | 262 | CAAATGTAAGAGGAAACTCAGAGGA | AGCAACAACTCCAGCTAATTGATAG | Â |
NtSAMDC | Â | 58 | 313 | ATTGGTTTTGAAGGTTTTGAGAAG | TCACGTCTTGTACTTTGAGAGACAG | Â |
NtSOD | Â | 58 | 238 | AGCTACATGACGCCATTTCC | CCCTGTAAAGCAGCACCTTC | Â |
Tubulin | Â | 58 | 163 | TCCAGGACAAGGAGGGTAT | CATCAACAACAGGCAACCTAG | Â |