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Figure 5 | BMC Plant Biology

Figure 5

From: Overexpression of PtrABF gene, a bZIP transcription factor isolated from Poncirus trifoliata, enhances dehydration and drought tolerance in tobacco via scavenging ROS and modulating expression of stress-responsive genes

Figure 5

Analysis of transactivation activity and ABRE binding of PtrABF. A. Growth of yeast cells transformed with either control vector (pGBKT7) or the fusion vector (pGBKT7: PtrABF) on SD/-Leu/-Trp, SD/-Ade/-His/-Leu/-Trp supplemented with 3-AT (0, 5 and 15 mM). B. Growth of the yeast cells on SD/-Ade/-His/-Leu/-Trp added with 15 mM 3-AT and 20 mM X-α-gal. C. Schematic diagrams of effector plasmids (E1 and E2) and reporter plasmids (R1 and R2) used for yeast one hybrid. D. Growth of yeast cells on SD/-His/-Leu/-Trp supplemented with (60 mM) or without 3-AT. Y1, Y2 and Y3 represent yeast cells transformed with E1 and R1, E2 and R1, E1 and R2, respectively.

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BMC Plant Biology

ISSN: 1471-2229

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