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Table 3 Experimental Functional Annotation of VvTPS Genes of the VvTPS-g Subfamily

From: Functional Annotation, Genome Organization and Phylogeny of the Grapevine (Vitis vinifera) Terpene Synthase Gene Family Based on Genome Assembly, FLcDNA Cloning, and Enzyme Assays

Functional Gene ID

Function

Access. #

FLcDNA ID

Nearest VvTPS gene model

Representative Products

%

VvPNLinNer1

(3S)- Linalool/(E)- Nerolidol syn

HM807391

PN25M6

VvTPS54

(3S)-Linalool

100*

VvPNLinNer2

 

HM807392

PN55M1

VvTPS56

  

VvCSLinNer

 

HM807393

CS2251F

VvTPS56

(E)-Nerolidol

100**

VvPNLNGl1

Linalool/(E)- Nerolidol/(E,E)-Geranyl linalool syn

HM807394

PNTPS271M2

VvTPS57

(3S)-Linalool

100*

VvPNLNGl2

 

HM807395

PNTPS271M5

VvTPS63

(E)-Nerolidol

100**

VvPNLNGl3

 

HM807396

PNTPS271M4

VvTPS58

(E,E)-Geranyl linalool

100***

VvPNLNGl4

 

HM807397

PN3M2

VvTPS61

  

VvGwGer

Geraniol syn

HM807398

Gw63YB3

VvTPS52

Geraniol

100

VvCSGer

 

HQ326231

CS5M2

VvTPS52

  

VvPNGer

 

HM807399

PN5L1

VvTPS52

  
  1. Terpenoids produced by individual VvTPS clones when incubated with GPP (*) or FPP (**) are listed. Where noted are trifunctional TPS capable of producing products also with GGPP (***). The tissue specific cDNA used to clone a particular gene is indicated in FLcDNA ID with either YB (young berry), F (flower), L (leaves) or M (mixed template consisting of stems, berries, flowers and leaves). FLcDNA ID in bold indicates clone came from cDNA of methyl jasmonate treated tissue. Clones with redundant functions are listed. Bolded VvTPS gene models indicate proof of transcript not previously known from the available ESTs.