Figure 8
Phylogeny of TPS-c, e, and f subfamilies. Phylogenetic relationships as determined by maximum likelihood analysis of the V. vinifera TPS-c,e, and -f subfamilies. Bootstrap values supported by ≥ 50% are designated * and those with values ≥ 80% are indicated with ^. TPS characterized in this paper are in teal and include VvCSENerGl and VvPNENerGl = (E)-nerolidol/(E,E)-geraniol linalool syn. VvTPS predicted from the 12-fold genome sequence assembly are in purple. TPS predicted by sequencing of the heterozygous Pinot noir are labeled with GenBank accession numbers (CAN....). TPS-c are all ent-copalyl diphosphate synthases - PtCPS = Populus trichocarpa (EEE81383), CmCPS1 = Cucurbita maxima (AAD04292), LsCPS1 = Lactuca sativa (BAB12440), SrCPS = Stevia rebaudiana (AAB87091), CmCPS2 = C. maxima (AAD04293), TaCPS = Triticum aestivum (BAH56560), OsCPS = Oryza sativa Japonica group (BAD42452). The following ent-kaurene synthases (Tps-e) are included in the analysis: PteKS = Populus trichocarpa (EEE88653), RceKS = Ricinus communis (EEF28689), HveKS = Hordeum vulgare subsp. Vulgare (AAT49066), LseKS1 = Lactuca sativa (BAB12441), SreKS = Stevia rebaudiana (AAD34295), ZmeKS = Zea mays (NP_001148059), OseKS1a = Oryza sativa Japonica group (AAQ72559). Abbreviations of the other included TPS are as follows: ZmFNF = Z. mays (E)-β-farnesene, (E)-nerolidol, (E,E)-farnesol syn (AAO18435), OsPim = O. sativa Indica group syn-pimara-7,15-diene syn (AAU05906), OS9Pim = O. sativa Japonica group 9b-pimara-7,15-diene syn (BAD54751), SHSanBer = Solanum habrochaites santalene/bergamotene syn (ACJ38409), SlPHS1 = S. lycopersicum β-phellandrene syn (ACO56896), NtTPS = Nicotiana tabacum terpene syn (AAS98912), AtGES = Arabidopsis thaliana geranyl linalool syn (NP_564772), CbSLin = Clarkia breweri (3S)-linalool syn (AAC49395), CbSLin2 = C. breweri (3S)-linalool syn 2 (AAD19840), AtTPS = A. thaliana tps (AAO85540).