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Figure 4 | BMC Plant Biology

Figure 4

From: The physical and functional borders of transit peptide-like sequences in secondary endosymbionts

Figure 4

Import properties of precursors with a truncated transit peptide. Wild type GFP with lysine at position four is stromally targeted with a truncated FcpD-TP (a), whereas GFP_K4E and GFP_K4A are not (b, c). GFP following spacer sequences is imported into the plastid only if the spacer exhibits positive charge (e), otherwise GFP localizes to the ER (d). Drawing on the right according to figure 1 with the synthetic spacer sequences in yellow (Spacer) representing a threefold incorporation (for spacer sequence see Table 1). Self-assembly GFP fluorescence is shown in the bottom line. Fragment 11 was cloned to the very N-terminus of GFP (see experimental procedures), fragment 1-10 was directed to the stroma by an AtpC-BTS. DIC = differential interference contrast, Plastid = chlorophyll autofluorescence, GFP = GFP fluorescence, Merge = merged plastid and GFP images. Scale bar = 10 μm.

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