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Figure 3 | BMC Plant Biology

Figure 3

From: The physical and functional borders of transit peptide-like sequences in secondary endosymbionts

Figure 3

GFP conveys transit peptide characteristics to a deficient targeting signal. Wild type GFP is partially transported into the PPC when following a TP with solely negative charges (a, e). Upon introduction of a lysine in the negatively charged TP, GFP carrying a lysine at position four is transported across the PPM (b), whereas GFP with glutamic acid at the N-terminus is not (c). A charge-neutral TP directs wildtype GFP into the PPC (d). Drawing on the right according to figure 1. In the bottom line, colocalization of the FcpD_K20E+R24E-eGFP (construct in topmost line) with a PPC-localized YFP (due to a sHsp70-BTS) is shown. DIC = differential interference contrast, GFP = GFP fluorescence, YFP = YFP fluorescence, Merge = merged images of GFP, YFP and chlorophyll autofluorescence, respectively. Scale bar = 10 μm.

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