Evolutionary relationships of the aconitate hydratase family in plants. The evolutionary history was inferred using the Neighbor-Joining method. The optimal tree with the sum of branch length = 4.42716789 is shown. Only significant bootstrap values are shown next to the branches. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. There were a total of 3737 positions in the final dataset. The different Aco subfamilies are indicated with labels close to the corresponding branches. Empty circles indicate dicot species, black circles designate monocot ones, squares mark the bryophyte P. patens. Species names are abbreviated as: Ath (Arabidopsis), Bdi (false purplebrome), Ccl (Clementine), Cli (lemon), Cpa (papaya), Cuc (cucumber), Glyma (soybean), Lpe (tomato), Mtr (barrel medic), Nta (tobacco), Osa (rice), Pav (peach), Ppa (P. patens), Pth (poplar), Rco (castor oil), Sbi (sorghum), Vvi (grapevine), and Zma (corn).