Variation and inheritance of iron reductase activity in the roots of common bean (Phaseolus vulgaris L.) and association with seed iron accumulation QTL

Table 2 Association of FRO loci and flanking markers with iron reductase activity (IRA) under iron limited (1 μM Fe) and iron sufficient (15 μM Fe) growth conditions and with seed iron concentration.

Locus¹	LG²	IRA (1 uM)		IRA (15 uM)		Fe-ICP³		Fe-AAS³
		LOD	R2	LOD	R2	LOD	R2	LOD	R2
FRO - SSR	6	0.33	.012	0.04	.002	1.23	.032	0.10	.004
Bng009	6	0.39	.014	0.05	.047	0.95	.026	0.11	.006
Bng027	6	0.02	.005	1.08	.002	1.15	.029	0.21	.004
FRO - CAPS (HindIII)	7	0.97	.017	0.05	.029	0.06	.002	2.31	.062
Bng060	7	0.14	.004	0.63	.027	1.02	.010	3.85*	.105*
Phs	7	0.12	.004	0.23	.018	1.46	.015	3.30*	.106*

1/The significance of successfully mapped FRO loci and their flanking markers are indicated in terms of LOD scores and R² values for iron reductase activity (IRA) and
2/LG (Linkage group) numbering according to Blair et al. (2003)
3/.Fe-ICP and Fe-AAS as described in materials and methods and Blair et al. (2009a), respectively.
* indicates significance at LOD threshold of 2.5 in single point regression analysis.

ISSN: 1471-2229