Molecular analysis of phosphomannomutase (PMM) genes reveals a unique PMM duplication event in diverse Triticeae species and the main PMM isozymes in bread wheat tissues

Table 1 PMM genes from B. distachyon, bread wheat and related Triticeae species, and rice ^a

Species	Gene	gDNA (bp)	cDNA (bp)	ORF	Protein (aa)
Brachypodium distachyon (Bd21) ^b	BdPMM	2696	750	+	249
Barley (Hordeum vulgare)	HvPMM-1	2185	750	+	249
(Betzes)	HvPMM-2	3263	771	-^c	-
Triticum urartu	TuPMM-A1	2228	750	+	249
(DV877 and IE29-1)	TuPMM-A2	2542	751	-^d	-
Aegilops tauschii	AetPMM-D1	2409	759	+	252
(AS67 and AS91)	AetPMM-D2	2348	756	+	251
Triticeae turgidum ssp. durum	TtPMM-A1	2227	750	+	249
(Langdon)	TtPMM-A2	2781	752	-^d	-
	TtPMM-B1	2256	750	+	249
	TtPMM-B2	2500	747	+	248
Bread wheat (Triticum aestivum)	TaPMM-A1	2225	750	+	249
(Xiaoyan 54, Chinese Spring)	TaPMM-A2	2778	751	-^d	-
	TaPMM-B1	2256	750	+	249
	TaPMM-B2	2500	747	+	248
	TaPMM-D1	2409	759	+	252
	TaPMM-D2	2346	756	+	251
Rice (Oryza sativa ssp. japonica) (Nipponbare)	OsPMM	2466 ^e	747 ^f	+	248

^a The genomic DNA (gDNA) and cDNA sequences include start (ATG) and stop (TGA) codons.
^b Plant materials from which PMM sequences were isolated.
^c ORF disrupted by premature stop codon.
^d ORF interrupted by frameshift mutation.
^e The chromosomal locus containing OsPMM genomic sequence is Os04g0682300.
^f The GenBank accession number for OsPMM cDNA is DQ442996.

ISSN: 1471-2229