Figure 5

Temperature-activity characteristics of the recombinant PMMs of bread wheat (TaPMM-A1, D1, and D2), barley (HvPMM-1), B. distachyon (BdPMM), and rice (OsPMM). The purified recombinant PMMs were each tested under three temperature conditions (24, 30 and 37°C), with mannose-1-phosphate as the substrate. The OD340 readings, recorded at 10 min after the start of the reaction, were used to compare the relative activity levels of the six PMMs under different temperatures. Means ± s.d. were each calculated using the results of triplicate measurements. Different letters above the bars suggest that the means differ significantly (P £ 0.05). The data shown are representative of three independent experiments.