Figure 3

Comparisons of the transcript levels of six TaPMM genes ( A1 , B1 , D1 , A2 , B2 , and D2 ) in Xiaoyan 54 organs collected at the seedling and bolting stages. The total RNA samples, extracted from the roots (R), seedling leaves (L), culms (C), flag leaves (FL), and immature spikes (IS), were converted into cDNAs through reverse transcription, followed by semiquantitative RT-PCR analysis with gene specific primers. The amplification of tubulin transcripts served as an internal control (for normalizing the cDNA levels of different reverse transcription samples and for checking the kinetics of PCR amplification). The numbers of amplification cycles for TaPMM and tubulin genes are shown on the right side of the graph. The data depicted are representative of three independent experiments.