AS5 transcript analysis. (A) Extent of the AS5 transgene (black bar) in relation to rrn5, trnR, and representative tobacco and tomato AS5 EST's (solid black arrows). Locations of the strand-specific RNA probes (P1-P3) used in (B) are shown above (dashed black arrows). ESTs are: 1 - tobacco AM826917, 479 nt; 2 - tomato AJ85186, 785 nt; 3 - tomato BI422136, 774 nt; 4 - tomato EST587142, 787 nt; 5 - tomato AJ832014, 696 nt. (B) 10% polyacrylamide gel blot analysis of AS5 in WT and AS5ox-1 (1) and AS5ox-2 (2). The probes used are shown at the top of the blot, and four WT bands (WT1-4) and an AS5ox-specific band (AS5ox) are indicated with arrows at the left. Two exposures are shown for the P2 probe to confirm bands WT1-3 and illustrate the abundance of WT4. Size standards are shown at the right.