The icme-like2-1 mutant showed increased sensitivity to ABA in seed germination. Seeds of wild-type and icme-like2-1 mutant were sown on 0.5× MS medium with different concentration of cis ABA and stratified at 4°C for 3 days, then transferred to growth room under conditions of 16 hr light/8 hr dark cycle at 22°C. Seed germination was scored every day since transferring. All assays were repeated at least three times with similar results. More than 108 seeds were used in each experiment. Asterisks represent significant differences from the wild type of P < 0.05 as determined by Student's t test.