Characterization of ICME and its homologs. (A) Multiple amino acid sequence alignment of ICME and its homologs was performed using Clustal W multiple alignment program. * represents the residues in that column are identical, : represents that conserved substitution have been observed, and · represents that semi-conserved substitution have been observed. Amino acids marked with red represent the putative carboxylesterase type-B serine active site, amino acids in the boxes represent the substrate binding pocket, and amino acids marked with # represent the catalytic triad. (B) Both ICME and its homologs belong to esterase lipase superfamily with conserved domain of carboxylesterase. (C) Phylogenetic relationships of ICME and its homologs, their homologs from Zea mays, Oryza sativa, Vitis vinifera, and Cleome spinosa as well as carboxylesterases from Arabidopsis. The figure showed an unrooted, bootstrap consensus plot, generated by the Neighbor-Joining method using MEGA4 program. The bootstrap values displayed are calculated based on 1000 replications.