Figure 1

RT-PCR analyses of all the studied ESTs. Expression was investigated by RT-PCR analysis on the tissues indicated above each lane. The ESTs amplified are indicated on the right. Male buds of two mm length represent the stage when meiosis starts in anthers. Female meiosis starts in eight mm long female buds. Further details concerning the flower development in S. latifolia are summarized in Additional file 6: Supplementary table S4 (according to Farbos et al. [47]). (A) ESTs expressed exclusively in male flower buds. (B) ESTs starting to be expressed in male flower buds earlier than in female flower buds. (C) ESTs expressed in leaves and flower buds of both sexes. (D) ESTs showing sex specific expression in all the studied tissues. Two of them (Serendip2, and Men-470) are showing male specific expression while CCLS79.1 gene shows female specific expression. Men-262 is included to illustrate that Serendip2 is amplified with the same pair of primers and it serves also as a proof of the sufficient quality of templates.