Instability of chromosome number and DNA methylation variation induced by hybridization and amphidiploid formation between Raphanus sativus L. and Brassica alboglabraBailey

Table 4 Different patterns of methylation in F₄ and F₁₀

Patterns			F₄		F₁₀		Number of sites	Ratio of different patterns (%)
			H	M	H	M
I		IA	-	+	-	+	74	58.29
		IB	+	-	+	-	28
II	A	IIA1	-	-	+	+	10	15.43
		IIA2	-	+	+	+	6
		IIA3	+	-	+	+	11
	B	IIB1	+	+	-	-	3	10.29
		IIB2	+	-	-	-	7
		IIB3	-	+	-	-	8
	C	IIC1	-	-	-	+	3	6.29
		IIC2	-	-	+	-	8
	D	IID1	+	+	-	+	8	5.71
		IID2	+	+	+	-	2
	E	IIE1	+	-	-	+	2	4.00
		IIE2	-	+	+	-	5

DNA methylation number of sites and ratio of different patterns. "- " stands for the absence of a band, and "+" stands for the presence of a band. H and M are the combinations of enzymes of EcoRI/HpaII and EcoRI/MspI, respectively.

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