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Figure 4 | BMC Plant Biology

Figure 4

From: Instability of chromosome number and DNA methylation variation induced by hybridization and amphidiploid formation between Raphanus sativus L. and Brassica alboglabraBailey

Figure 4

Examples of MSAP profiles showing various types of locus-specific DNA methylation inheritance and variation in F 1 hybrid and their parents (a) and different types of locus-specific DNA methylation inheritance and variation in F 4 and F 10 , and the F 4 and F 10 DNA methylation patterns (b,c). (a). Primer combination is EcoRI+ ATT/HpaII (MspI) + ACC. Amplification results of HpaII-digested genomic DNA of R. sativus L. (Lane 1), B. alboglabra Bailey (Lane 3) and F1 hybrids (Lane 5) Amplification results of MspI-digested genomic DNA of R. sativus L. (Lane 2), B. alboglabra Bailey (Lane 4), and F1 hybrids (Lane 6). (b) and (c). Primer combinations are EcoRI + AGG/HpaII (MspI) + GAT and EcoRI + AGG/HpaII (MspI) + TCG, respectively. Arrows show variations of the various methylation patterns. H: HpaII, M: MspI.

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BMC Plant Biology

ISSN: 1471-2229

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