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Table 3 Identification success based on "best close match" tools.

From: DNA barcoding of the Lemnaceae, a family of aquatic monocots

 

psbK-psbl

trnH-psbA

matK

atp-atpH

rpoB

rpoCl

rbcL

psbK-psbl + atp F-atpH

trnH-psbA + atp F-atpH

matK+ atpF-atpH

rpoB+atp F-atpH

rpoCl + atp F-atp H

rbcL + atpF-atpH

Correct

72(84.7%)

66(82.5%)

49(77.77%)

78(92.85%)

62(77.5%)

60(70.58%)

60(70.58%)

79(94.04%)

71(89.87%)

/

77(91.66%)

77(91.66%)

77(91.66%)

Ambiguous

8(9.41%)

11(13.75%)

10(15.87%)

3(3.57%)

12(15.0%)

21(24.7%)

21(24.7%)

0(0.0%)

3(3.79%)

/

2(2.38%)

4(4.76%)

4(4.76%)

Incorrect

5(5.88%)

2(2.5%)

4(6.34%)

3(3.57%)

6(7.5%)

4(4.7%)

2(2.35%)

5(5.95%)

5(6.32%)

/

5(5.95%)

3(3.57%)

3(3.57%)

No match

0(0.0%)

1(1.25%)

0(0.0%)

0(0.0%)

0(0.0%)

0(0.0%)

2(2.35%)

0(0.0%)

0(0.0%)

/

0(0.0%)

0(0.0%)

0(0.0%)

Threshold

22.12%

4.01%

2.62%

2.96%

2.57%

0.44%

0.38%

22.16%

2.08%

/

2.44%

1.77%

1.67%

  1. "best close match" was analyzed by TAXONDNA program [31] with single region or two-region combinations. The ecotypes was classified into correct, ambiguous, incorrect and no match group. The group number was shown in each well. Number in bracket indicates percentage in all barcoding ecotypes. matK + atpF-atpH was not counted due to the small number of sequence comparison done for matK. Percentage in the bracket was calculated by dividing each item by all tested sample.