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Table 3 Identification success based on "best close match" tools.

From: DNA barcoding of the Lemnaceae, a family of aquatic monocots

  psbK-psbl trnH-psbA matK atp-atpH rpoB rpoCl rbcL psbK-psbl + atp F-atpH trnH-psbA + atp F-atpH matK+ atpF-atpH rpoB+atp F-atpH rpoCl + atp F-atp H rbcL + atpF-atpH
Correct 72(84.7%) 66(82.5%) 49(77.77%) 78(92.85%) 62(77.5%) 60(70.58%) 60(70.58%) 79(94.04%) 71(89.87%) / 77(91.66%) 77(91.66%) 77(91.66%)
Ambiguous 8(9.41%) 11(13.75%) 10(15.87%) 3(3.57%) 12(15.0%) 21(24.7%) 21(24.7%) 0(0.0%) 3(3.79%) / 2(2.38%) 4(4.76%) 4(4.76%)
Incorrect 5(5.88%) 2(2.5%) 4(6.34%) 3(3.57%) 6(7.5%) 4(4.7%) 2(2.35%) 5(5.95%) 5(6.32%) / 5(5.95%) 3(3.57%) 3(3.57%)
No match 0(0.0%) 1(1.25%) 0(0.0%) 0(0.0%) 0(0.0%) 0(0.0%) 2(2.35%) 0(0.0%) 0(0.0%) / 0(0.0%) 0(0.0%) 0(0.0%)
Threshold 22.12% 4.01% 2.62% 2.96% 2.57% 0.44% 0.38% 22.16% 2.08% / 2.44% 1.77% 1.67%
  1. "best close match" was analyzed by TAXONDNA program [31] with single region or two-region combinations. The ecotypes was classified into correct, ambiguous, incorrect and no match group. The group number was shown in each well. Number in bracket indicates percentage in all barcoding ecotypes. matK + atpF-atpH was not counted due to the small number of sequence comparison done for matK. Percentage in the bracket was calculated by dividing each item by all tested sample.